Flp-in t-rex
WebNov 22, 2016 · We next generated stable isogenic HEK293 Flp-In T-REx cell lines with tetracycline-inducible expression, resulting in the expression of 140 protein phosphatases ( Table S1; Figures S1 A and S1B), 114 of which were previously shown to display endogenous mRNA expression in HEK293 ( Uhlén et al., 2015; Figure 1 A; Table S1). WebAug 20, 2024 · The Flp-In system integrates a vector into cell lines at a specific genomic locus, but has not been used for integration of more than one gene. Here we present a modification of the Flp-In...
Flp-in t-rex
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WebUpon isolation of DPCs by PxP, samples were analysed using western blotting using the indicated antibodies. d HeLa T-REx Flp-In SPRTN-ΔC cells were complemented with YFP, YFP-SPRTN-Strep variants as indicated (wildtype (WT), E112Q (EQ), R185A (ZBD*)) and treated as shown in Fig. , before DPCs were isolated using PxP and analysed by …
WebAs a first step to Flp-In T-rex cell lines, you have to create the so called host cell line, which is generated through stable random integration of two plasmids pFRT/lacZeo … Webjor components of the Flp-In™ T-REX™ System include: • A Flp-In™ target site vector, pFRT/lacZeo, for generation of a Flp-In™ host cell line containing an integrated FRT site. …
WebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebOur lab has bought Invitrogen Flp-In T-rex cells line 1 year ago and we now got our clones. The problem is that the promotor seems to be very strong and our protein aggregated so we want to reduce the expression level. The manuscript of them recommended to use 0.1-1µg/ml Tetracycline or similar conc. of Doxycycline to induced the cell line.
WebApr 26, 2024 · Flp-In T-REx 293 cells expressing c-IBD-SBP-YAP1C were incubated in DPBS containing 10 mM 3-AT and supplemented or not with 1 mM H 2 O 2. After 10 min, free thiol groups were blocked with NEM, and the samples were processed as depicted in panel A and subsequently subjected to immunoblotting with rabbit pre-immune serum …
WebAs a first step to Flp-In T-rex cell lines, you have to create the so called host cell line, which is generated through stable random integration of two plasmids pFRT/lacZeo (introducing the FRT... phonetics as a branch of linguisticsWebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. After 48 hours transfection, I... how do you test for mrsa infectionWebApr 4, 2013 · Flp - In T-REx- 293 Cells Generation of Flp - In Expression Cell Lines Maximum size of insert that can be used Selection with Hygromycin Transfection protocol for Flp - In Jurkat cells Transfection protocol for Flp - In -CHO cells Efficiency of Flp - In integration versus random integration phonetics assignment 8-2WebApr 11, 2024 · The Flp-In TM 293 T-REx TM pcDNA.5/FRT/TO inducible Tet-ON expression system was used to create stable, inducible cell lines, whereby the Ku70-BioID2 fusion protein (wild-type, Ku70 S155A, or Ku70 S155D) or the Ku70 NLS-BioID2 fusion protein was stably integrated in the T-REx 293 cell line and under the control of a doxycycline … phonetics aspirationWebApr 7, 2024 · Flp-in-T-REx 293 cells expressing either GFP or 3xFlag-tagged human SRRM4 were induced with 1 ug/ml for 24 h . Total RNA was extracted using the Illustra RNAspin Mini Isolation kit (GE Healthcare). Total RNA was extracted using the Illustra RNAspin Mini Isolation kit (GE Healthcare). how do you test for mcasWebDescription. Once Flp-InT-REx host cell line containing integrated FRT site has been created, subsequent generation of Flp-InT-REx cell lines expressing gene (s) of interest … how do you test for mold toxicityWebInducible Flp-In T-Rex 293 cells were generated according to the manufacturer’s protocol (Thermo Fisher Scientific). The selection was performed with DMEM supplemented with 15 μg/ml blasticidin and 100 μg/ml hygromycin B Gold (InvivoGen) 48 h after transfection and continued until the expression of the gene of interest was induced by ... phonetics assignment answers