Graphene based gene transfection
WebJan 10, 2014 · Successful gene therapy requires efficient and safe gene vectors that protect DNA from nuclease degradation as well as facilitate DNA uptake with high transfection efficiency [139], [140]. Graphene has been explored for applications in gene delivery, gene–drug co-delivery and protein delivery with good supportive literature available. WebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of …
Graphene based gene transfection
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WebMar 1, 2011 · Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for … WebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene transfection.
WebAug 18, 2024 · Deoxyribonucleic acid (DNA) vaccines are a promising cancer immunotherapy approach. However, effective delivery of DNA to antigen-presenting cells (e.g., dendritic cells (DCs)) for the induction of an adaptive immune response is limited. Conventional DNA delivery via intramuscular, intradermal, and subcutaneous injection by … WebTo evaluate the transfection efficiency of NGQDs, we delivered mRNA into HeLa cells with NGQDs. As shown in Figure 4 a, the NGQDs formed complexes with mRNA at a 20:1 …
WebNov 22, 2016 · Graphene based gene transfection. Nanoscale 2011; 3: 1252–1257. Article CAS Google Scholar Prencipe G, Tabakman SM, Welsher K, Liu Z, Goodwin AP, Zhang L et al. PEG branched polymer for ... WebJun 10, 2013 · Graphene oxide (GO) has been extensively explored in nanomedicine for its excellent physiochemical, electrical, and optical properties. ... 1 Jiangsu Key Laboratory for Carbon-Based, Functional Materials & Devices, ... remarkably enhanced plasmid DNA transfection efficiencies induced by the NIR laser are achieved using NGO-PEG-PEI as …
WebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene …
WebJun 18, 2024 · Abstract. Functionalized graphene oxide (GO) nanoparticles are being increasingly employed for designing modern drug delivery systems because of their high degree of functionalization, high surface area with … phil woodcroftWebDec 1, 2024 · Graphene and its derivatives, as sheet-like carbonic nanomaterials, have been progressively utilized in various biomedical fields. Currently, applications of … tsinghua university aspiWebOct 13, 2014 · Graphene based biosensors make use of two strategies-1) probe molecule on graphene interacts with the analyte (FRET based ... Zhang S, Liu Z (2011) Graphene based gene transfection. Nanoscale 3: 1252-1257. Zhou L, Wang W, Tang J, Zhou JH, Jiang HJ, et al. (2011) Graphene oxide noncovalent photosensitizer and its anticancer … phil wood congressWebDec 4, 2024 · The objective of this study is to design a graphene-based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene … tsinghua university auditoriumWebGene therapy has been found to hold tremendous potential for the treatment of cancers beyond conventional methods. 30–32 Nonviral vector provides safer methods without the potential side effects of viral transfection, such as immunogenicity and carcinogenesis. 33 GO has been considered an ideal vector for gene delivery. 34,35 In this study ... philwooddesignWebJan 21, 2016 · During PhD work, I extensively involved on the development of chitosan based novel non-viral vectors for gene therapy application. I developed polyamidoamine (PAMAM) dendrimer conjugated chitosan copolymer through various chemical methods and showed very high transfection efficiency. All my research works published in reputed … tsinghua university architectureWebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also … tsinghua university alumni