Mouse raw264.7 cells
Nettetfor 1 dag siden · Furthermore, T cells from NO mice retain differentiation of osteoclast precursors of RAW 264.7 macrophages ex vivo. Our data reveal that HFD is not a deleterious diet; however, the induction... NettetRAW‑Dual™ cells stably express two reporter genes encoding SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase . SEAP expression depends on the …
Mouse raw264.7 cells
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NettetThe RAW264.7 cells, pMac from the healthy or immunosuppressed BALB/c mice with positive phagocytosis of T. marneffei were 91.63%±2.59, 89.26%±3.48, 65.75%±2.81 ( B, C, D ), respectively. Pre-treatment with anti-CD11b, the positive phagocytosis was 35.45%±3.15 ( E ). NettetStudies with the mouse monocyte cell line RAW 264.7 and mouse bone marrow indicated that MCP-1 promotes chemoattraction of pre-osteoclasts, and enhances RANKL …
Nettet12. apr. 2024 · RAW264.7 cells were seeded in a 6-well plate at a density of 1 × 10 6 cells per well. Twenty-four hours later, the RAW264.7 cells were pretreated with NDC (70 … Nettet7. des. 2024 · RAW264.7 cells were cultured and treated as indicated, then washed for three times with PBS. The cells were fixed with 4% paraformaldehyde for 15 min and penetrated with 0.1% Triton X-100 for 10 min. The cells were blocked with PBS containing 5% bovine serum albumin (BSA) for 1 h, then with anti- TNF-α (CST, United States) at …
Nettet13. apr. 2024 · The RAW264 cells (a mouse leukemic monocyte cell line; ECA85062803) were purchased from RIKEN BRC through the National BioResource Project of the …
Nettet12. apr. 2024 · It was indicated that once the RAW264.7 cells were stimulated by LPS, iNOS would be released in the cells, and then a large amount of NO, that was mediated by iNOS, would become synthesized in the cells, and cause severe inflammation [ 24 ].
NettetOsteoclasts are the unique cell type capable of resorbing bone. The discovery of the TNF-ligand family member, RANKL, has allowed more reliable study of these important … grainne hallinanNettetFunctionally, the viability of macrophage RAW264.7 cells was significantly affected by exosomes at a high concentration (160 µg/mL). The production of IL-12, TNF-α and IFN … grain kennettNettet3. des. 2024 · RAW264.7 cells were seeded in 6-well dishes the day before transfection at 0.5 × 10 6 cells/well. Cells were transfected with 2 μg of DNA using Lipofectamine LTX, according the manufacturer’s ... grain levy loginNettet1. des. 2024 · As a result, HML was not cytotoxic to RAW264.7 cells, but rather, the cell viability of RAW264.7 cells treated with HML was increased (Fig. 1 D). In addition, we compared the degree of macrophage activation of HML with ginseng, one of the representative traditional herbal medicines related to immune enhancement through the … grainne sullivan tuslaNettetFlow cytometric analysis of IL-27 p28 (IL-30) expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-γ (Cat. No. 554587; 24 h; Left Plot) … grain mite ovaNettet16. des. 2024 · In co-cultures of RAW264.7 and 3T3-L1 cells, silybin (50 and 100 μM), quercetin (100 μM), formononetin (50 and 100 μM), kaempferol (100 μM), naringin (100 μM), diosmetin (50 and 100 μM), apigenin (100 μM), and atorvastatin (100 nM, positive control) inhibited adipogenesis and lipid accumulation performed by Oil Red O staining … grainne jolleyNettetマウス細胞株 • 副腎 • 骨髄／胸腺 • 胸部 • 結腸／胃 • 胚線維芽細胞 • 皮膚 • 造血系 • 腎臓 • 肝臓 • 肺 • リンパ • 筋芽 • ニューロブラストーマ • 卵巣／精巣 • 紡錘細胞 MTAの締結について ご購入頂く際に、「Material Transfer Agreement（MTA）」を締結する必要がございます 。 こちらからダウンロードください。 CLI社 細胞製品 使用目的確認書 ※企業 … grain myruss