Phospho-ampk substrate motif
WebWe systematically explored the effects of the AMPK-MFN2 signaling axis on dynamic changes of mitochondria, autophagy and MAMs in response to energy stresses. Results Energy stress promotes MAM formation, autophagy and mitochondrial fission WebMay 1, 2015 · Here, we report an affinity proteomicstrategy for the discovery and validation of AMPK targets using an antibody to isolate proteins containing the phospho-AMPK substrate recognition motif from hepatocytes that had been treated with pharmacological AMPK activators.
Phospho-ampk substrate motif
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WebJul 9, 2024 · a p-AMPK substrate motif-specific antibody showing phosphorylation of GST-RUNX2-WT but not in lanes of serine-specific mutant of RUNX2-S118A, and GST control … WebApr 7, 2024 · AMPK is rapidly activated following any stress that triggers a lowering of the intracellular adenosine 5′-triphosphate (ATP)/adenosine 5′-diphosphate (ADP) ratio, …
WebJan 10, 2024 · We found that the phospho-AMPK substrate motif antibody, which is more specific than the phospho-Ser antibody, was able to detect MFF phosphorylation. However, the phospho-AMPK substrate... We would like to show you a description here but the site won’t allow us. WebAMPK is a highly conserved sensor of intracellular adenosine nucleotide levels that is activated when even modest decreases in ATP production result in relative increases in …
WebFeb 15, 2011 · To date, people have gathered large amounts of phosphorylation data, making it possible to perform substrate-driven motif discovery using data mining techniques. Results: We describe an algorithm called Motif-All that is able to efficiently identify all statistically significant motifs. The proposed method explores a support … WebJan 10, 2024 · Further analysis using an AMPK consensus phosphorylation motif indicated that 32 of these sites are likely direct AMPK phosphorylation sites. We validated one uncharacterized protein, ARMC10, and demonstrated that the S45 site of ARMC10 can be phosphorylated by AMPK both in vitro and in vivo.
WebJan 10, 2024 · The phosphosites that were upregulated in WT cells but did not meet these rules and did not have sequences similar to the conserved AMPK substrate motif constituted group 2 ( n = 77). These sites may be regulated by the AMPK-dependent signaling pathways but are not directly phosphorylated by AMPK.
WebNov 3, 2015 · We provide an AMPK motif matrix and a pipeline to predict additional AMPK substrates from quantitative phosphoproteomics datasets. As AMPK is emerging as a … optus contractor inductionWebDec 23, 2010 · Many in vivo substrates of AMPK not only conform to this motif but also bind to the phospho-binding protein 14-3-3 inducibly upon phosphorylation by AMPK. We therefore screened for proteins that bound to recombinant 14-3-3 in wild-type (WT) but not AMPK-deficient cells, and only under conditions of energy stress, when AMPK would be … optus country zonesWebMay 1, 2015 · AMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Although it is best known for its effects on … portsmouth airport parkingWebAMP-activated protein kinase (AMPK) is a conserved energy sensor that plays roles in diverse biological processes via phosphorylating various substrates. Emerging studies … optus corporate officeWebMar 18, 2024 · Although the phosphorylation motif is not a perfect fit, it has been previously shown that AMPK can potently phosphorylate its substrate without the canonical motif, such as ULK1 [Citation 63–65]. Importantly, while WT GST-TFEB is efficiently phosphorylated by AMPK in vitro , a truncation of the 36 C-terminal residues led to a dramatic ... optus coverage checkWebAnalysis of phospho-AMPK substrate motif antibody immunoprecipitates by MS/MS. (A, B) Primary hepatocytes were isolated from male C57BL/6 mice (12-week-old). Hepatocytes were incubated with ... portsmouth aerial viewWebTable: PTMScan®Results,Phospho-AMPK Substrate Motif (LXRXXS*/T*), SILAC Study design: Human non-small cell lung cancer (H1650) cell line; LysC/post-IAP trypsin digest; … optus coverage longreach